The requirement is for a custom 10X single cell library preparation and sequencing of 16 samples prepared using the “tissue fixation and dissociation for chromium fixed RNA profiling” protocol (CG000553). Approximately, 7,500-12,500 cells are estimated to be recovered from sequencing.
The acquisition of a Chromium cell library will provide the program an extensive opportunity to characterize single cell response in a 3D lung model for Coronavirus and other high impact respiratory viruses.Project requirements: The Chromium cell library must be brand-name or equal to the items listed in Attachment 1.
The National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health (NIH) intends to negotiate on an other than full and open competition basis with 10X Genomics, Inc. to procure The Chromium TM i7 Multiplex kit, single cell library and chips.
FOB: DestinationBrand name requirements:ChromiumTM Single Cell 3' Library & Gel Bead Kit v2, 16 rxns CAT# 120237 1 eachChromiumTM Single Cell 5' Library & Gel Bead Kit, 4 rxns CAT# 1000014 2 eachChromiumTM Single Cell A Chip Kit, 48 rxns CAT# 120236 1 eachChromiumTM Single Cell V(D)J Enrichment Kit, Human B Cell, 96 rxns CAT# 1000016 1 eachChromiumTM Single Cell V(D)J Enrichment Kit, Human T Cell, 96 rxns CAT# 1000005 1 eachManufactured by:
Performance/functional specifications of product: Product will be used to prepare single cell library preparation with a high capacity automated single cell sequencer. Salient characteristics: product must be capable of preparing single-cell library preparation to a level that is usable for high throughput sequencing.
(v) The Items required are:Product Code 1000009 Chromium Single Cell A Chip Kit, 16 Rxns Qty 1Product Code 120267 Chromium Single Cell 3' Library and Gel Bead Kit, 4 rxns Qty 1Product Code 120263 Chromium Single Cell Controller & Accessory Kit, 12 mo.
(vi) Generically, this acquisition consists of cell libraries to characterize single cell response in a 3D lung model to SARS-CoV2 for a SARS-CoV-2 project.(vii) Delivery shall be to:The National Center for Advancing Translational Sciences (NCATS)9800 Medical Center DriveRockville, MD 20850Delivery shall be within 30 days after receipt of order.
