The requirement is for a custom 10X single cell library preparation and sequencing of 16 samples prepared using the “tissue fixation and dissociation for chromium fixed RNA profiling” protocol (CG000553). Approximately, 7,500-12,500 cells are estimated to be recovered from sequencing.
Answer: The price quote shall include all accessories needed to set up and use the instrument and sufficient reagents and kits to allow for training and provide for at least 96 Illumina i7 Multiplexing reactions, 16 library preparation reactions, and chips with associated reagents sufficient for 48 reactions.Contact Information: Warren P.
The National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health (NIH) intends to negotiate on an other than full and open competition basis with Psomagen, Inc. for cDNA clean-up and library preparation from single cell libraries generated from studies of COVID-19-recovered individuals on a sole source basis due to continuity protocols.The statutory authority for this sole source requirement is 41 U.S.C. 1901
and visualization software; open sourcedata analysis tools for rapid processing of Illumina sequencing data• Enables single cell 3’ counting of mRNA at 100X reduction in price compared to commonly usedsingle cell platforms• Prepares up to 80,000 cells for single cell library preparation in 7 minutesQuantity:5x Chromium Next GEM Chip G Single Cell Kit, 48 rxns5x Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1, 16 rxnsQuantity:SevenDELIVERY
• Provides stand alone and turn-key analysis pipelines and visualization software; open source data analysis tools for rapid processing of Illumina sequencing data • Enables single cell 3’ counting of mRNA at 100X reduction in price compared to commonly used single cell platforms • Prepares up to 80,000 cells for single cell library preparation in 7 minutes Quantity: 5x Chromium Next GEM Chip G Single Cell Kit, 48 rxns 5x Chromium Next
No other company offers a single cell preparation platform with the same capacity as 10X Genomics, which can prepare up to 80,000 cells for a single library and 8 libraries in a single run. 10X Genomics also provide open source data analysis tools for analysis pipeline and data visualization (Cell ranger and Loupe cell browser).
alone and turn-key analysis pipelines and visualization software; open source data analysis tools for rapid processing of Illumina sequencing data • Enables single cell 3' counting of mRNA at 100X reduction in price compared to commonly used single cell platforms • Prepares up to 80,000 cells for single cell library preparation in 7 minutes • Requires low capital outlay • Recovers up to ~65% of cells (typically 50%) • Low doublet rate (~0.9% per
