The EPA is interested in the contractor measuring multiplexed transcriptomic changes for up to 50,000 protein coding genes using purified RNA, cDNA libraries, or tissue lysates (where compatible with the technology) as inputs.
The EPA is interested in the contractor measuring multiplexed transcriptomic changes for up to 50,000 protein coding genes using purified RNA, cDNA libraries, or tissue lysates (where compatible with the technology) as inputs.
with existing portfolio of TaqMan assays for gene expression, genetic variation, gene regulation, and other quantification experiments using Microfluidic Array Plate (MAP) technology.Capabilities: (if applicable): __This machine will be used to analyze environmental DNA and other molecular samples.This purchase will be made utilizing Simplified Acquisition Procedures in accordance with:Federal Acquisition Regulation (FAR) Part 13.106-1(b) for purchases
Assays must be compatible with the BioRad CFX connect qPCR machine currently housed in the USGS Alaska Science Center Molecular Ecology Laboratory. Additionally, assays must include 96-well plates pre-loaded with reagents, master mix that may be added to wells with multichannel pipettes, and appropriate reaction controls.
The reagents must also detect the HgbS mutation in the beta globin gene. The reagents must be compatible with a BioArray Array Imaging System™ (AIS™ 400), with assay results interpreted, analyzed, and reported through BioArray Solutions Information System (BASIS™) software.Reag Box,PreType BeadCheck Kit;Salient characteristics: Quality control material for the Precise Type HEA BeadChip Kit.
with the same luciferase reporter gene, firefly luciferase, present within our existing pseudo virus stocks;*Substrate shall exhibit reproducibility, signal-to-noise ratio, and sensitivity equal to Promega #E4550 Luciferase 1000 Assay System;* Shall be capable of measuring firefly luciferase in single-tube or multi-well plate luminometer equipped with auto-injector;*Substrate shall provide linear results over at least eight orders of magnitude of
(vi) USDA, Agricultural Research Service (ARS), Animal Parasitic Diseases Laboratory (APDL) is requesting a custom gene codeset for use with multiplex nucleic acid hybridization technology.*See attached Specification Sheet for additional information* (vii) Performance location: USDA, ARS, APDL, 10300 Baltimore Ave, BLDG 1040, RM 103, BARC-EAST, Beltsville, MD 20705.
