Search results for: hybridoma cell lines

*Clarification: For the existing cell lines (which consist of the bulk of the samples to be stored), reproducing and freezing of the cell lines (tasks 1 and 2) is not required as they have already been stored in a frozen state and must simply be maintained in that state.Quantity: 154 vials of hybridoma cell lines.The contractor shall continue to maintain the hybridoma cell lines under liquid nitrogen, during the entire period of the contract.

services,qualified personnel, material, equipment, and facilities, not otherwise provided by the Government as needed to perform the Statement of Work.Specific Requirements:Multiple positive clones (2 vials of frozen hybridoma cells for each positive clone) per target.0.1-0.5mg purified antibody (>90% purity by SDS-PAGE) for each positive cloneValidated antibody pair (1 pair, not guaranteed): 2 frozen vials of hybridoma cells and 10mg purified antibody

The United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Midwest Area (MWA), Acquisitions and Personal Property/Fleet Office in Peoria, Illinois on behalf of the National Center for Agricultural Utilization Research’s (NCAUR) in Peoria, Illinois received approval to make a sole source award in accordance with FAR 13.106-1. This document sets forth the justification for a sole source award which is permitted under FAR 13.106-1(b)(1)(i).

The Firm Fixed PO will include the following:1. 34 HYBRIDOMA CELL LINES FOR ASCITES PRODUCTION: 25 BALB/C MOUSE PRODUCTION RUNS PER HYBRIDOMA CELL LINEUSDA ARS, WRRC, FTDP research has an essential requirement for 34 hybridoma cell lines that need ascites production. They would need 25 mice per hybridoma cell line and do not need any protein A/G purification.

SUB-CLONING OF AN EXISTING HYBRIDOMA CELL LINE Solicitation ID/Procurement Identifier: 12505B19P0056 Ultimate Completion Date: Thu Aug 27 17:00:00 GMT 2020

Resulting hybridomas will be screened in a high throughput ELISA format to generate a panel of hybridoma cell lines secreting target-specific antibodies. Follow-up screening will include screening in the presence of a human serum matrix. Hybridomas of interest will be scaled in culture and cryopreserved for subsequent Partner-based confirmation assessment.

Funds are only available for use for the line item to which they are obligated.SPECIAL ORDER REQUIREMENTPRODUCT FEATURES/SALIENT CHARACTERISTICSThe following product features/characteristics are required for this requirement:Ten individual, unique hybridoma cell lines must be produced from animals previously inoculated for NCI with antibodies purified and sequenced against phospho-mEAK-7-Ser440.Each cell line shall include the following features:Antibody