PROXIMITY LIGATION LIBRARY PREPARATION AND SEQUENCING Solicitation ID/Procurement Identifier: 12905B24Q0023 Ultimate Completion Date: Tue Apr 30 17:00:00 GMT 2024
AMPLICON LIBRARY PREPARATION AND SEQUENCING Solicitation ID/Procurement Identifier: 1333MJ23BNRMA0050 Ultimate Completion Date: Fri Sep 20 17:00:00 GMT 2024
The library preparation kit should include a variety of steps that include enzymatic fragmentation, size selection, end-repair, a-tail and ligation step in the same reaction. The process should allow including dual index at the 5 and 3 prime end for later sample identification. Also, the library preparation kit should be designed to construct libraries with a low DNA input (~50ng) with master-mixed reagents ready to use.
Library preparation must include:Library preparation according to Tag-Seq protocol (https://github.com/z0on/tag-based_RNAseq).Quantification of prepared libraries by Agilent Bioanalyzer or TapeStationProven track record of success with RNA from coral samplesIllumina sequencing: Must be able to complete Tag-Seq sequencing, including:NovaSeq 6000
The USDA, ARS, MWA, Plant Genetics Research Unit, Columbia, MO has a requirement/need for Illumina library preparation and sequencing.
Nucleic Acid Sequencing and Library Preparation to gather Sequence Data; The Contractor must provide total RNA library preparation and sequencing services from samples provided by the Government in accordance with the Statement of Work (SOW).
Answer: The price quote shall include all accessories needed to set up and use the instrument and sufficient reagents and kits to allow for training and provide for at least 96 Illumina i7 Multiplexing reactions, 16 library preparation reactions, and chips with associated reagents sufficient for 48 reactions.Contact Information: Warren P.
