THIS SERVICE IS TO CONDUCT WHOLE-GENOME METHYLOME ANALYSIS OF 96 DNA SAMPLES USING ILLUMINA 850K EPIC V2 ARRAYS IN THE SAME PROCESS AND PLATFORM AND ANALYTICAL PIPELINE AS THE OTHER SAMPLES THAT WERE ... Solicitation ID/Procurement Identifier: 12305B23Q0193 Ultimate Completion Date: Tue Jun 25 17:00:00 GMT 2024
MULTIPLEXED RNA SEQUENCING FOR 15 PRE-POOLED LIBRARY SAMPLES USING ILLUMINA HISEQ400 (PE150) TECHNOLOGY. A TOTAL OF 288 INDIVIDUAL SAMPLES WILL BE COMBINED INTO POOLED LIBRARY SETS TO BE DIVIDED ACROSS 15 SEQUENCING LANES. Solicitation ID/Procurement Identifier: 1333MF19PNFFP0240 Ultimate Completion Date: Tue Oct 15 17:00:00 GMT 2019
ANALYSIS OF 96 DNA SAMPLES USING THE ILLUMINA EPIC METHYLATION ARRAY Solicitation ID/Procurement Identifier: 75N95D19P00179 Ultimate Completion Date: Fri Feb 28 18:00:00 GMT 2020
In addition, determination of SCFA concentration is also required for part of the same intestinal content samples used for sequencing.The contractor will perform DNA extraction and library preparation for 137 intestinal content samples providing quality control data.Sequencing of libraries will be performed using Illumina platform with 2 x150 base pair-end reads and the Illumina Novaseq2 instrument for whole-genome shotgun sequencing with a mean depth
The project workflow shall consist of the following:Preparation of cDNA libraries for both mRNA-/lncRNA-Seq and small RNA-SeqRNA quality control for all samples before and after library preparationPerformance of RNA-Seq workflowsmRNA-/lncRNA-Seq for 216 samples using an Illumina platformSmall RNA-Seq for 96 samples, including clustering prior to sequencing, using an Illumina platformQuality control of all readsAlignment of reads to reference genomeData
The Contractor shall provide all labor, material and equipment necessary to perform scanning of 2,129 existing DNA samples that were collected from subjects enrolled in the Chinese centers of the AsiaLymph study. The Contractor shall scan DNA samples using disposable Illumina GSA chips to ensure the scientific comparability of the new data with the previously generated data.
Diagenode is a leading global provider of complete solutions for epigenetics research, biological samples preparations and diagnostics assays. The company has extensive experience using the Illumina platforms and the Infinium Methylation EPIC Array Kit. Diagenode can provide a quick turnaround time for processing the 480 samples needed. This project was initiated in August of 2021 and Diagenode provided the methylation service.
