THE GOAL OF THIS PROPOSAL IS TO IDENTIFY NOVEL EARLY BIOMARKERS IN PLASMA AND URINE TO DETECT AND MONITOR THE PROGRESSION OF A NUMBER OF PHYSIOLOGICAL DISTURBAN...

THE GOAL OF THIS PROPOSAL IS TO IDENTIFY NOVEL EARLY BIOMARKERS IN PLASMA AND URINE TO DETECT AND MONITOR THE PROGRESSION OF A NUMBER OF PHYSIOLOGICAL DISTURBANCES DUE TO PROLONGED MICROGRAVITY AND CO2 EXPOSURE AS EXPERIENCED BY CREW MEMBERS ON LONG DURATION MISSIONS. THESE PHYSIOLOGICAL MANIFESTATIONS INCLUDE: (1) VISUAL IMPAIRMENT/INTRACRANIAL PRESSURE (VIIP); (2) SUB-CLINICAL OR ENVIRONMENTALLY INDUCED CARDIOVASCULAR DISEASE; (3) MUSCLE ATROPHY AND DECREASED MUSCLE STRENGTH; AND (4) BONE LOSS. TARGETED AND UNTARGETED METABOLOMICS WILL BE APPLIED TO PLASMA AND URINE COLLECTED LONGITUDINALLY FROM STUDY PARTICIPANTS UNDERGOING A 30 DAY SIX-DEGREE HEAD-DOWN BED REST COMBINED WITH AMBIENT 0.5% CO2. WE WILL FOLLOW-UP METABOLOMICS WITH A NOVEL CELL-BASED METABOLIC MAMMALIAN ORGAN SYSTEM ASSAY ("ORGANS ON A PLATE") TO ADDRESS HOW THESE METABOLITES AFFECT PHYSIOLOGICAL PROCESSES AT THE CELLULAR AND ORGAN LEVEL. THE PROPOSED RESEARCH WILL DIRECTLY ADDRESS THE INTEGRATED RESEARCH PLAN GAPS INCLUDING CV8, M6, OSTEO5, VIIP3, VIIP13, CNS2.
Solicitation ID/Procurement Identifier: NNX16AG03G
Ultimate Completion Date: Tue Dec 31 18:00:00 GMT