Automated Cell Culture Processing System

expired opportunity(Expired)
From: Federal Government(Federal)
HHS-NIH-NIDA(AG)-SS-20-75N95020Q00138

Basic Details

started - 27 May, 2020 (about 3 years ago)

Start Date

27 May, 2020 (about 3 years ago)
due - 11 Jun, 2020 (about 3 years ago)

Due Date

11 Jun, 2020 (about 3 years ago)
Bid Notification

Type

Bid Notification
HHS-NIH-NIDA(AG)-SS-20-75N95020Q00138

Identifier

HHS-NIH-NIDA(AG)-SS-20-75N95020Q00138
HEALTH AND HUMAN SERVICES, DEPARTMENT OF

Customer / Agency

HEALTH AND HUMAN SERVICES, DEPARTMENT OF (26486)NATIONAL INSTITUTES OF HEALTH (10750)NATIONAL INSTITUTES OF HEALTH NIA (1852)
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This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement.This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition.  It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract.  Responses will not be considered as proposals or quotes.  No award will be made as a result of this notice.  The Government will NOT be responsible for any costs incurred by the respondents to this notice.  This notice is strictly for research and information purposes only.Background:This custom designed Automated Cell Culture Processing System is an important component of our efforts to systematically analyze induced pluripotent
stem cells (IPSC) associated with neurodegenerative disorders. Currently, we have been manually processing our IPSCs for smaller scale projects but in order to increase the throughput of our data we are looking to upgrade to an automated system. Purpose and Objectives:Automated Cell Culture Processing Systems mimic the manual cell culture process to maintain and expand multiple cell lines in a range from 6-well, 12-well, single well, and 384-well formats. The production of cells in large quantities and in consistent quality is a critical step for the implementation of cell-based high throughput screens (HTS). Current cell production for HTS is done manually, which leads to suboptimal batch-to-batch consistency, is a contamination-prone process, it has limited scalability, and lacks the needed industrial scale reproducibility. This leads to inconsistencies in the results of the screens, reduced productivity and increased cost. This is particularly challenging when using stem cell and stem cell-derived cells because of the long duration of cultures, frequent media exchange steps to introduce differentiation media, and the high number of steps in the processing protocols. Automated tissue culture enables cell production with greater reproducibility and productivity. Flexible processing capabilities of our IPSCs will enable the provision of larger quantities of cells daily for cell-based assays while simultaneously growing smaller volumes of different cell lines for basic research and development activities.Project requirements:Salient Characteristics:The system must be capable of culturing multiple cell lines with cell line specific protocols. It must be able to operate unattended, enabling more effective use of valuable resources. The system must be able to culture delicate or complex cell lines such as iPS cells and primary cells. The system must be capable of maintaining stock cell lines for multiple projects. The system must be equipped with automated system that decaps/caps screw     capped tubes and provides barcode reading of 1-D and 2-D barcodes. The system must be equipped with an automated centrifuge for centrifugation of deep well plates to pellet down cells. The system must have a heater-shaker with 3.0 mm orbit as well as a tilting of    plates in the y-axis for media aspiration. The system must be equipped with a 4-degree media storage system. The system must be equipped with a 37C degree warming delivery system to enable media dispensing (30 - 5,000μL) for multiple and simultaneous media exchanges. The system must be able to send system updates via email. The system must automatically back up valuable information to remote media. The system must allow users to generate custom formatted reports as well as adjust protocol programming. The system must be able to generate assay-ready plates for cell-based screening and assay development. The system must be capable of processing 6, 24, 96 and 384-well standard plates and also 24 and 96-well insert plates at consecutively and/or congruently. The system must be equipped with a barcode reader on the plating module to track   plates during operation. The system must enable direct imaging and counting of cells in 1 well, 6-well, 12-well and 384-well, confluence measurement and enhanced QA of cells and has to include required lenses for detailed fluorescent imaging. The system must allow life cycle management support including operator training, preventative maintenance, and system upgrades. Installation, training and delivery shall be performed by the contractor. 12 month warranty with 6 month preventive maintenance must be included. Warranty option for three (3) additional years after expiration of initial 12 month warranty must be included.Anticipated delivery:Delivery date of 12 months or less after receipt of completed purchase orderOther important considerations:All deliverables required under this contract shall be packaged, marked and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition.Capability statement /information sought:Respondents must provide clear and convincing documentation of their capability of providing the equipment specified in this notice.The respondent must also provide their DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc., pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements.Finally, respondents are also encouraged to provide a general overview of the respondent’s opinions about the difficulty and/or feasibility of the potential requirement, and any other information regarding innovative ideas or concepts that may be applicable.Information Submission Instructions Respondents should provide responses accordingly: (1) submit information electronically via email. No telephone, hard copy, or facsimile responses will be accepted; (2) format capability statements using Microsoft Word or Adobe PDF including attachments, resumes, charts, etc. Use single space, 12 point font minimum and 8 1/2 x 11 page size; (3) organize material in such a manner that clearly identifies and addresses capability requirements (4) capability statement should not exceed ten (10) single sided pages including references; (6) responses should be received no later than June 11, 2020 at 4:00 PM EDT; (7) include respondents' technical and administrative points of contact, Including names, titles, addresses, telephone and fax numbers, and e-mail addresses; and (8) send responses to this notice via email to eric.mckay@nih.gov.Disclaimer and Important Notes:This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization’s qualifications to perform the work.Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in beta.SAM.gov. However, responses to this notice will not be considered adequate responses to a solicitation.Confidentiality:No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).

Bethesda ,
 MD  20892  USALocation

Place Of Performance : N/A

Country : United StatesState : MarylandCity : Bethesda

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Classification

naicsCode 334516Analytical Laboratory Instrument Manufacturing
pscCode 6640Laboratory Equipment and Supplies